DNA
BsaI ->

Part:BBa_K1362424:Design

Designed by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha   Group: iGEM14_Heidelberg   (2014-10-07)

BsaI restriction site for RFC[105] cloning


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1


Design Notes

This part is only the sequence of a restriction site. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[105] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.


Source

This standard restriction site sequence is used as part of the iGEM team Heidelberg 2014's Intein Toolbox [1]. All associated standard sequences can be reviewed in RFC[105]

References

[1] [http://2014.igem.org/Team:Heidelberg iGEM team Heidelberg 2014]

[2] [link RFC[105]]